FUW TRENDS IN SCIENCE & TECHNOLOGY JOURNAL
(A Peer Review Journal)
e–ISSN: 2408–5162; p–ISSN: 2048–5170
keywords: Acute effect, glyphosate, heteroclarias fingerlings, acute toxicity and fish toxicology
This study was carried out to determine the acute effect of glyphosate on water quality parameters and some antioxidant response of heteroclarias fingerlings. A range finding test was conducted six times in order to get reliable values that could be used for the definitive test. In the definitive test, the toxicant introduced at varying concentrations of 0.00 mg/l, 5.40 mg/l, 7.20 mg/l, 9.00 mg/l, 10.80 mg/l and 12.60 mg/l for 96 h in a renewal bioassay procedure showed that the 96 h LC50 was 6.838 mg/l. There was a sharp increase in some water quality parameters such as pH, temperature, total dissolved solids, electrical conductivity and total hardness after exposure of the toxicant for 96 h, while dissolved oxygen and alkalinity decreased. There was significant difference in gill catalase activity of heteroclarias fingerlings exposed to different concentration of glyphosate for 96 h (P<0.05), heteroclarias exposed to 5.40 mg/l of glyphosate had the highest gill catalase activity (1.323±0.00 U/mg protein) and 0.00 mg/l with the lowest gill catalase activity (0.003±0.00 U/mg protein), gill peroxidase activity also differed significantly (P<0.05), heteroclarias fingerlings exposed to 9.00 mg/l of glyphosate had the highest and 0.00 mg/l with the lowest gill peroxidase activity. Liver catalase and peroxidase activity of heteroclarias fingerlings also differed significantly at vary concentrations of glyphosate (P<0.05), heteroclarias exposed to 12.60 mg/l showed the highest liver peroxidase activity level, i.e. the antioxidant response of heteroclarias fingerlings was concentration dependent.
American Public Health Association (APHA) 1995. Standard Methods for the Examination of Water and Waste Water. 19th ed. Washington DC, pp.1,100. American Public Health Association (APHA) 1989. Standard Methods for the Examination of Water and Waste Water. 17th edition. Washington DC, pp. 1,268. Aysel S, Ergul BK, Tulay A& Ekoloji P 2010. Trend of organ-wise change in selected antioxidant enzyme activity in spotted scat from Cochin backwaters. Adv. App. Sci. Res., 19(76): 8-14. Cassilas E & Smith LS 1977. Effect of stress on blood coagulation and haematology in rainbow trout (Salmogairdneri).J. Fish Biol., 10:481-491. Dill GM, Sammons RD, Feng PCC, Kohn F, Kretzmer K, Mehrsheikh A. Bleek M, Honegger JL, Farmer D, Wright D & Haupfear EA 2010. Glyphosate: Discovery, development, applications and properties. In: Nandula VK editor. Glyphosate Resistance in Crops and Weeds: History, Development & Management. New York: Wiley, pp. 1-33. Doherty VF, Ogunkuade OO& Kanife UC 2010. Biomarkers of oxidative stress and heavy metal levels as indicators of environmental pollution in some selected fishes in Lagos, Nigeria. Am-Eur. J. Agric Environ Sci., 7(3):359-365. Kandemir, S., Dogru, M.I., Orun, I., Dogru, A., Altas, L., Erdogan, K., Orun, G. & Polat, N. 2010. J. Animal Vet. Adv.,9(3):617-622. Lind, O.T. 1979. A Hand Book of Common Methods of Water Analysis for Limnology. C.V. Moshy Publishers, St. Loius, USA, p. 69. Luck H 1974. In: Methods in Enzymatic Analysis2 (ed.Bergmeyer), Academic Press New York, p. 885. Lushchak, V.I. 2011. Environmentally induced oxidative stress in aquatic animals. Aquat Toxicol, 101:13-30. Mozdzer TJ, Hutto CJ, Clarke PA & Field DP 2008. Efficacy of imazapyr and glyphosate in the control of non-native Phragmites australis. Restorat Ecol., 16(2):221-224. Nogueira BL, Rodrigues ACF, Trídico CP, Ossa CE & De Almeida EA 2010. Environ Monitor Assess, DOI 10.1007/s10661-010-1785-9. Obaiah J & Usha AR 2012. Trend of organ-wise change in selected antioxidant enzyme activity in spotted scat from Cochin backwaters. Int. J.Pharm. Sci, 4(5): 303-310. Papchenkova GA, Golovanova IL & Ushankova NV 2009.The parameters of reproduction, sizes and activities of hydrolases in Daphnia magna Straus of successive generations affected by Round-up herbicide. Inl. Water Biol., 2(3):286-291. Radovanovic TB, Slavica S, Mitic B, Branka R, Perendija SG, Despotovic PSZ, Cakic PD & Zorica SS 2010. Arch. Biol. Sci. Belgrade, 62(1):97-105. Reddy JK, Suga T, Mannaerts GP, Lazarow PB & Ubramani SS 1995. Peroxisomes: Biology and Role in Toxicology and Disease. New York: Annals of the New York, p. 801. Skea JG, Neuberger J, Symoola G Aylesworth T & Miceoli J 1987. The toxicity of Ortho Diquat to thefry to several species of warm water fish. Technical Report No. 87-1 (BEP), Department of Environmental Conservation, Albany, NY. Solbe JF 1995. Fresh water In: Handbook of Ecotoxicology (Edited byPeter Collins) Black Well Science Ltd. Osneymeed OX 20EL,p. 683. Van Vuren JHJ 1986. The effects of toxicants on the haematology of Labeounbratus. Comparat Biochem. Physiol., 83:155-159
